Plasmid

Part:BBa_K4357006

Designed by: Di Yu   Group: iGEM22_UM_Macau   (2022-10-12)


J23100-mCherry-pS1C3

In the direction for our gene expression there is a promotor (BBa_J23100) from Constitutive promoter family member which has the strongest strength among BBa series of promoters in the Anderson promoter library, RBS (BBa_B0034) which is the ribosome binding site, mCherry gene which is our fluorescent reporter gene, 6xhis which is our his tag, double terminator (BBa_B0015) which is the terminator, BioBrick suffix and his Oberon terminator. In the opposite direction, the cat promotor controls the CmR gene which is the selective marker, carrying chloramphenicol resistance., followed by Iambda t0 terminator. There is also a replication origin, pUC19-derived pMB1 (copy number of 100-300 per cell).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 3005
    Illegal suffix found in sequence at 957
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3005
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 958
    Illegal PstI site found at 972
    Illegal NotI site found at 776
    Illegal NotI site found at 965
    Illegal NotI site found at 3011
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3005
    Illegal XhoI site found at 785
    Illegal XhoI site found at 1989
    Illegal XhoI site found at 2881
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 3005
    Illegal suffix found in sequence at 958
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 3005
    Illegal XbaI site found at 3020
    Illegal SpeI site found at 958
    Illegal PstI site found at 972
  • 1000
    COMPATIBLE WITH RFC[1000]


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